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KMID : 1041220030450020090
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Journal of Sericultural and Entomological Science
2003 Volume.45 No. 2 p.90 ~ p.95
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Identification of C3G(cyanidin-3-glucoside) from Mulberry Fruits and Quantification with Different Varieties
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Kim Hyun-Bok
Kim Sun-Lim
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Abstract
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This study was carried out to identify of C3G (cyanidin-3-glucoside) from mulberry fruits and quantify with different varieties. C3G of mulberry fruits was extracted with 1％ HCl-MeOH and purified with open column (5${\times}$90cm) which filled with Amberlite IRC-50 ion exchange resin. The $\lambda$max ranges of the purified C3G on UV/vis spectrum were 516nm and 280nm. Also, molecular weight of C3G from mulberry fruits by LC-Mass was determined as 449. From above results, we concluded that anthocyanin pigment of mulberry fruits was C3G only. The cyanidin-3-glucoside (C3G) was separated and quantified by High Performance Liquid Chromatography (HPLC) system using a Nova-Pack C$\_$18/ column. Mean content of the 35 tested accessions was 0.89％. Also fruity characteristics as well as C3G content to select the desirable mulberry varieties for the production of fruit were researched and analyzed. We selected three suitable varieties such as ’’’’’’’’Susungppong’’’’’’’’, ’’’’’’’’Kangsun’’’’’’’’, and ’’’’’’’’Jeolgokchosaeng(Chungpuk)’’’’’’’’
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KEYWORD
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C3G, Cyanidin-3-glucoside, Anthocyanin, Mulberry fruit, Fruity characteristic
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